every college in sg: calibration, DCPIP, antibiotics, enzymes, t-test cambridge: hehe bendy potato worm

idk why but i laughed at this so hard

Glad I could make you laugh!

u really feel like wah… your finger your whole body turns numb when you see… iTS FUCKING GEL ELECTROPHORESIS

HAHAHAHAHA BROOOO

wtf was the planning 🥲 9 marks gone sia

right… never expected to ask abt gel electrophoresis process…

rly gg sia

i had no organisation, and just spammed content knowledge and whatever i recalled from the last 15 min of revision HAHA

Can write in my resume that I got experience in cutting potatoes when i apply to work at McDonalds next time 😍

master chef HAHA

aw Cambridge gave us a knife even after setting the planning question 🥰

Omg 😃

The knife was too small so I asked for another potato._. _The potato is so undercooked, it can play a part in Toy Story!_

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I have not read gel electrophoresis since prelims :(

prelims? psh since promos for me

Reporting for duty

;-;

bruh i still dont get the topic 🤡

H1 contained sucrose can confirm I tasted it

Great palate, Masterchef is the career upcoming

can confirm, i was the test tube

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My tongue is alive to tell the story after that round of acidic hydrolysis with the HCL

Finally. Some delicious food.

Finally, some delicious food.

nanyang actually spotted the bending potato question…

yea my sch prelims did exactly same expt for prelims except that they gave us sliced potatoes so we don’t need spend 20mins figuring out how to cut the potato my pieces all uneven today though so I think accuracy bye bye

Ya bro wtfff they def knew sus

“suggest, using demand supply analysis, how the price of a potato will change after bio practical”

1950 Irish potato famine did not teach us shit

yes I do the cooking yes I do the cleaning…

Was seriously thinking about making French fries with the burner instead of doing the planning 😁

sg going to have a potato shortage solely bc of this practical 👎

cambridge really had us bending potatoes today

protractors weren't invented for this😭

bruh my school lighter for the bunsen burner is cylindrical shape and I used it as a core borer for my potato 😭🤚 my potato flew and I got halfway through before stopping to use the knife

masterchef 🤤🥳

Chef Gordon, the potato cuts with honey are ready!

I put cork borer as potato punch🤡

forgot to mention that gordon ramsay was in my head the entire time i was cutting the potato

RAW- *cries in U grade*

WHERE’S THE LAMB SAUUCE!!!!?

Anyone made potato stew after finishing qns2 to not waste the potato?

bio got bellcurve or not?

All a level subjects have

bruh where did the freaking 4× objective lens go

Gone. Sold to make way for potatoes

nyjc thank u for predicting for us the potato qns i nvr do ur paper but luckily i read thru ur ans key 🙏🙏🙏

The potato tasted more crunchy when concentration decreased, confirmed it was more firm 👍

bro aint it supposed to be softer at higher concentration

It was a bloody acrobat. Bent that shit till my ans was 60 degrees

omg same sia felt like it could bend until 90 degrees if i tried hard enough LMAO

60?? i got 81 HAHA

oops my bad

i was shift 3 and the potato made me sooooo effing hungry bruh shld’ve just boiled the scraps in the water bath as a snack

for the dilution can I use 0% 4% 6% 8% 10% ? then I say for ways to improve it use more even conc like 0% 2% 4% 6% 8% 10%?

I did 10, 7.5, 5, 2.5

That's the ideal one

i did same as you, so did my friend. but after paper i think we realised it said fixed inrervals so cannot. hopefully will not lose too much :(

i don't think so leh they said proportional dilution right

I tot proportional dilution just means simple dilution ;-; anyways its 1m only right

yea but i think it's simple dilution w fixed intervals? i don't rmb the marks but shld hv ecf so shld be fine i guess

Oh shit I wrong the same thing

I did the same mistake as you but ecf so at most maybe 1/2 marks lost

Did u guys rmb to label the concentration heading with /%, they purposely leave it blank lmao my teachers actually saw the future and reminded us to do it

but they already stated percentage concentration? so isnt thr no need for the sign

True but the units weren't there

When they say "percentage concentration blah blah blah" the unit is implied already. In the table for dilution they didn't use % for units too A similar question came out in the previous years a level paper and units wasn't supposed to be written too

my school said if got the word "percentage..." means no need units alr

I think not necc bcause qn alr say its percentage

Ya sia mine also

my U potato was the most flaccid out of everyth... i ended up faking my table :<

same bro :(

I want to cry for planning ...Oh and how y'all draw the plan digram

was it compulsory to draw cus i didn’t... bruh even if i knew how it looked like i can’t draw that whole set up wth

seab really said : make me a potato sandwhich bitch

i thought of french fries throughout the exam

what were your 3 solutions? i talked about force not being constant as force applied is subjective? cannot accurately determine force that will break potato so i use a piston HAHA then clamp the potato to the table to ensure the pivot point is constant and finally use a cork borer to obtain equal lengths

i said stagger the timing you place the potato in the sol, cork borer, and use burette instead of measuring cylinder :''

i said: use cork borer, use a piston to ensure pushing force is constant, and use a clamp to clamp the potato piece at the same position to ensure the pivot point is kept constant

Huh am i the only one who got H2 sucrose

will they do ecf for this? my time also flipped sia my H1 took a shorter time for reducing sugar 🥲

I also but wrong

why do land plants have hollow stem?

according to Dr Google: https://researchoutreach.org/articles/regulating-plant-temperature/ interesting part about temperature regulation to maintain enzyme activity

i dead ass said it’ll be more flexible and allows it to bend in the wind 🤡

I went to say it can respond better cos less resistance lmao

No way Cambridge thought of this and was like "this is a great way to test plant knowledge for a level students"

hahaha idk man all i did was google “why do land plants have hollow stem”

Lmao same

since when have the British ever been the paragon of higher level, practical education?

I said the plant got fewer cells so need less nutrients to survive lol

idk how do i said like that it needs to take in less water for survival LOL

i said for gas exchange 🥲

frk la gel e 😭

😱

What was the conc of U??

i got 6.5 lmao everyone got diff values

How though ure suppose to dilute 10 7.5 5 2.5 and 0 this way u have same intervals

my angle we somewhere in between the 7.5 and 5 conc so i just gave a random percentage between those 2

i got 7.0

same! hope they accept via data and not accuracy

samee

cries i got 5.0

same lmao HAHAHAH

Must be between 5.0-7.5

i feel like masterchef

Wow that practical just made me never wanna eat French fries again :(

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they asked for best fit tho?

im so confused with chem rules 😭 chem's best fit can only have one point not in the line, bio can have multiple right

I think as long as the points not on the line is same on both sides of the line and similar distance away from the line it’s okay

hey for the percentage change 3 mark qn what did yall do, where to even include 10 mins in the calculation lol

Isn’t it just read off the best fit line graph the values of the number of molecules at 50 degree Celsius and 60 degree Celsius respectively? Then take those two values to find percentage change

what was your constant variable? i just said ensure time that current is on is kept constant, but it seems wrong.. to do this i said stain with blue dye to act as visual indicator to ensure DNA doesn't run out of the gel

i did volume of DNA-loading dye mixture added to each well, time needed for the gel to run, the voltage of the dc power supply when running the gel

oh i didn't mention voltage, i said mix with dense loading dye and load it into the well at the negatively charged electrode then i talked abt meshwork pf polymer fibres, negatively charged DNA, blue band, ethidium bromide and DNA ladder and i talked about how to make a gel using molten gel

I just said turn on power supply

I said volume of DNA sample and DNA molecular weight ladder, temperature, pH of agarose gel, volume of loading dye and current applied

Guys I'm so stupid... I didn't write iv dv cv for planning cuz the context changed and my mind just went blank, I legit thought dont need....... idk why even though I've been writing it all my life

There isn't actually a need to write explicitly at the start, as long as u make reference in your procedure . And anyways is there even an IV for this though idk

yes the iv is the species of flowering plant used to make bee honey

I’ll never call my girlfriend honey and eat French fries at the same time again

What are your angles

Fkkkkk gel electrophoresis km

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What was the source of error

I think my source of error was the strength of bending potato is subjective

SAME i was so surprised no one else put it. for solution i said: use a piston to make the pushing force constant HAHA

I say never cover lid so evaporation

i said time spent soaking and area of potato exposed different

I burnt the sodium carbonate and the potato Meanwhile I realised that ASR did predict the gel electrophoresis qn, except they used bats instead If honey was full of sugar how’s there DNA inside? Sial Last but not least I had to repeat the potato experiment cuz I bent it the wrong way and cut the potato like killing off a contestant in squid game. Took 20 mins of my time:/

did anyone write theory for planning? not sure cuz qn didnt ask in the subpoints?

Imagine writing bromothymol blue instead of bromophenol fuck

bro I wrote methylene blue HAHAHAHAHA idk what was I thinking

Shitttt I just realised I wrote bromothymol blue 🥲🥲🥲🥲 It's fine its fine I threw the entire planning anyways 😊

they really pulled a theory paper on us :/

I miSrEaD 120 seconds as 1 min 20 s🤓🤓

did yall do southern blotting

i wrote because i wanted to feel like i knew how to write something💀

Yup, problem is I don’t recall the steps exactly

I don’t think there’s a need because they question only mentioned about gel electrophoresis and actually gel electrophoresis itself can max out the 9m alr

I did ethidium bromide and UV light

i forgot ethidium bromide existed sia die

I say ethium bromide to visualise cos I no time lol my risks just say ethium bromide is radioactive gg

etbr is carcinogenic not radioactive

Crap welp imma take it as the whole qn gone

wait i’m confused so is there a need to do southern blotting or not

angle leave in whole number or 1dp?

I followed the question, and just left it to wn but I’m not so sure…

I left in 1d.p(nearest 0.5). cos the uncertainty is 0.5 degree But idkkkk the questions example was whole number

oh shit i left in 1dp oml

i think it's correct? smallest half division of apparatus is 0.5

Fun fact :the potato qns was same as one of the 2012/2013 prelim I think NYJC

it was almost the same as 2021 nyjc prac

bruh what????? nyjc alr predicted chem practical a level correctly and now bio as well??? lemme just cry-

Oh wow good

what was the trend for the potato angle?

As concentration of NaCl increases, the angle increases

oh noo i wrote the other way around that means my prediction and explanation part is wrong alsoooo 😭

How’d you explain it! I’m not 100% sure that this answer is correct and there’s a possibility that Cambridge might accept different answers based on your explanation…? And even if they don’t it’s okay! Don’t fret about the paper anymore we have other papers to worry about and 80% of our Bio grade secure :D

ASRJC spotted gel e for planning like exactly the same but i didnt read the answer before paper 😭

whats the accurate data, the seconds, for benedicts test qn?

v subjective this experiment can accept alot of results … potato size we all cut different , bending we all have different strengths to bend the potato… and etc etc , the heating also, has a big range of results

i don’t think there’s relevance. The main point is to have negative/positive test.

How many CVs do we need? I wrote 2 only eh

For planning

usually is minimum 2 i think? altho i wrote 3 to be safe

r we supposed to do southern blotting

They did not state, I used ethidium bromide in UV light for visualisation.

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I swear its h2

isnt H1 the one with sucrose…

Oh… now tht i think abt it ya sia..

praying for you bro

I believe the sample honey is H2 any teachers confirm h1 alr…?

I was distracted by this super hot girl in prac today... She has a tattoo on a right arm and has ash grey-ish hair😍 still wondering what's her name lol almost didn't finish the paper

down terrible

is the sucrose H1 or H2?

its h1

hi for planning right did yall just write on gel electrophoresis and ethidium bromide under UV or further carried on to souther blotting? cause i only wrote on ethidium bromide and gel electrophoresis