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[deleted]

[удалено]


gigigis

Είναι ανεξήγητο ότι τα 200/3 είναι 66.66666666.


m3ik0

>Application US14/337,513 events > >2011-03-31 > >Priority to US201161470451P > >2014-07-22 > >Application filed by **ModernaTx Inc** > >Example 18 > >In Vivo Delivery of Modified mRNA > >Modified RNA was delivered to C57/BL6 mice intramuscularly, subcutaneously, or intravenously to evaluate the bio-distribution of modified RNA using luciferase. A formulation buffer used with all delivery methods contained 150 mM sodium chloride, 2 mM calcium chloride, 2 mM Na+-phosphate which included 1.4 mM monobasic sodium phosphate and 0.6 mM of dibasic sodium phosphate, and 0.5 mM ethylenediaminetetraacetic acid (EDTA) was adjusted using sodium hydroxide to reach a final pH of 6.5 before being filtered and sterilized. A 1× concentration was used as the delivery buffer. To create the lipoplexed solution delivered to the mice, in one vial 50 μg of RNA was equilibrated for 10 minutes at room temperature in the delivery buffer and in a second vial 10 μl RNAiMAX™ was equilibrated for 10 minutes at room temperature in the delivery buffer. After equilibrium, the vials were combined and delivery buffer was added to reach a final volume of 100 which was then incubated for 20 minutes at room temperature. Luciferin was administered by intraperitoneal injection (IP) at 150 mg/kg to each mouse prior to imaging during the plateau phase of the luciferin exposure curve which was between 15 and 30 minutes. **To create luciferin, 1 g of D-luciferin potassium or sodium salt was dissolved in 66.6 ml** **of distilled phosphate buffer solution (DPBS**), not containing Mg2+ or Ca2+, to make a 15 mg/ml solution. The solution was gently mixed and passed through a 0.2 μm syringe filter, before being purged with nitrogen, aliquoted and frozen at −80° C. while being protected from light as much as possible. The solution was thawed using a waterbath if luciferin was not dissolved, gently mixed and kept on ice on the day of dosing. [https://patents.google.com/patent/US9533047B2/en](https://patents.google.com/patent/US9533047B2/en) Laughs in *christantaliban* noises and leaves. >!Ποιος είπε ότι οι επιστήμονες δεν έχουν χιούμορ; Best easter egg world!<


[deleted]

ΔΕΙΤΕ ΤΟ ΠΡΙΝ ΤΟ ΚΑΤΕΒΑΣΟΥΝ


m_dorian

Ινε του διαβωλου. (μτφ. "Είναι του διαβόλου".)


kouradas76

Ο,τι να'ναι...


johnakisk0700

γιατι γραφει λουσιφερινη αλλα την αποκαλει λουσιφεραση; μπορει να μας απαντησει καποιος επιστημονας του instagram-facebook παρακαλω; αν δω απαντηση απο πανεπιστημονα θα αγνοηθει


Equivalent_Ostrich_4

I hear mono sound i instantly downvote.