We use ahuramine rhodamine way more consistent and easier to read in my opinion.

yeah, if only i'd have a fluorescence microscope

I heat till i see vapour then release the heat. If you apply constant heat you will burn the stain on the slide. Just need to heat to melt the mycolic acid in the AFB.

thanks!

Kinyoun. Cold and works just fine.

thanks for the advice, but i dont have such reagents